TERT antibody

Catalog No. ABIN6266278

Product Details anti-TERT Antibody

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TERT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: TERT Antibody detects endogenous levels of total TERT.

Predicted Reactivity: Pig,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TERT

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-TERT antibody (ABIN6266278)

Zhang, Chen, Wang, Yang, Li, Wang, Liu, Ye: "Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase." in: Oncotarget, Vol. 7, Issue 26, pp. 39302-39315, (2018) (PubMed).

Bai, Liu, Xu, Qiu, Wang, Zheng: "Expression profiles of stemness genes in gastrointestinal stromal tumor." in: Human pathology, Vol. 76, pp. 76-84, (2018) (PubMed).

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Alternative Name: TERT (TERT Products)

Synonyms: CMM9 antibody, DKCA2 antibody, DKCB4 antibody, EST2 antibody, PFBMFT1 antibody, TCS1 antibody, TP2 antibody, TRT antibody, hEST2 antibody, hTRT antibody, chTERT antibody, Tert antibody, ATTERT antibody, F5E19.190 antibody, F5E19_190 antibody, telomerase reverse transcriptase antibody, tert antibody, TR antibody, est2 antibody, tcs1 antibody, telomerase antibody, tert-A antibody, tp2 antibody, trt antibody, xTERT antibody, telomerase reverse transcriptase antibody, Telomerase Reverse Transcriptase antibody, Tertp antibody, Telomerase catalytic subunit antibody, telomerase reverse transcriptase L homeolog antibody, TERT antibody, trt-1 antibody, Tert antibody, tert antibody, CMU_003490 antibody, VDBG_04487 antibody, GL50803_16225 antibody, tert.L antibody

Background:

Description: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

Gene: TERT

Molecular Weight: 120kDa

Gene ID: 7015

UniProt: O14746

Pathways: Telomere Maintenance