GATA4 antibody (pSer105)

Catalog No. ABIN6267526

Product Details anti-GATA4 Antibody

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Binding Specificity: pSer105

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-GATA4 (Ser105) Antibody detects endogenous levels of GATA4 only when phosphorylated at Serine 105.

Predicted Reactivity: Pig,Bovine,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human GATA4 around the phosphorylation site of Ser105.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4 (GATA4 Products)

Synonyms: gata-4 antibody, xgata4 antibody, xGATA-4 antibody, MGC81427 antibody, GATA4 antibody, GATA-4 antibody, Gata4 antibody, gata4 antibody, ASD2 antibody, VSD1 antibody, Gata-4 antibody, gata antibody, GATA binding protein 4 antibody, GATA binding protein 4 L homeolog antibody, glutamyl-tRNA(Gln) amidotransferase subunit A antibody, gata4 transcription factor antibody, GATA binding protein 4 S homeolog antibody, GATA4 antibody, gata4.L antibody, gata4 antibody, gatA4 antibody, Gata4 antibody, gata4.S antibody

Background:

Description: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).

Gene: GATA4

Molecular Weight: 44kDa

Gene ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis