MAP2K3 antibody (pSer189)

Catalog No. ABIN6267536

Product Details anti-MAP2K3 Antibody

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Binding Specificity: pSer189

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-MKK3 (Ser189) Antibody detects endogenous levels of MKK3 only when phosphorylated at Serine 189.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human MKK3 around the phosphorylation site of Ser189.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Alternative Name: MAP2K3 (MAP2K3 Products)

Synonyms: MAPKK3 antibody, MEK3 antibody, MKK3 antibody, PRKMK3 antibody, SAPKK-2 antibody, SAPKK2 antibody, AW212142 antibody, Prkmk3 antibody, mMKK3b antibody, Mkk3 antibody, mitogen-activated protein kinase kinase 3 antibody, mitogen activated protein kinase kinase 3 antibody, MAP kinase activator XMEK3 antibody, MAP2K3 antibody, Map2k3 antibody, map2k3 antibody

Background:

Description: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

Gene: MAP2K3

Molecular Weight: 40kDa

Gene ID: 5606

UniProt: P46734

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2