Integrin alpha 4 (ITGA4) (pSer1027) antibody Primary Antibody
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- Target
- Binding Specificity
- pSer1027
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Clonality
- Polyclonal
- Conjugate
- Un-conjugated
- Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Integrin α4 (Phospho-Ser1027) Antibody detects endogenous levels of Integrin α4 only when phosphorylated at Ser1027
- Cross-Reactivity
- Human, Mouse (Murine)
- Purification
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- Immunogen
- A synthesized peptide derived from human Integrin alpha4 (Phospho-Ser1027)
- Isotype
- IgG
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- Target
- Alternative Name
- Integrin alpha4 (ITGA4 Antibody Abstract)
- Background
Description: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
Gene: ITGA4
- Molecular Weight
- 115kDa
- Gene ID
- 3676
- UniProt
- P13612
- Pathways
- Integrin Complex
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- Application Notes
- WB 1:1000-3000, IHC 1:50-1:200
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C.Stable for 12 months from date of receipt
- Expiry Date
- 12 months
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