MED27 antibody (Internal Region)

Catalog No. ABIN6275724

Product Details anti-MED27 Antibody

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED27 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: MED27 Antibody detects endogenous levels of total MED27.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MED27, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: MED27 (MED27 Products)

Synonyms: CRAP34 antibody, CRSP34 antibody, CRSP8 antibody, TRAP37 antibody, CG1245 antibody, Dmel\\CG1245 antibody, Med27 antibody, Trap37 antibody, dCRSP34 antibody, dCrsp34 antibody, dTRAP37 antibody, med27 antibody, crsp8 antibody, zgc:66302 antibody, MED27 antibody, DDBDRAFT_0216764 antibody, DDBDRAFT_0266847 antibody, DDB_0216764 antibody, DDB_0266847 antibody, 1500015J03Rik antibody, 2310042P07Rik antibody, AA682045 antibody, Crsp8 antibody, D2Ertd434e antibody, RGD1564993 antibody, DKFZp459I1912 antibody, CRSP complex subunit 8-B antibody, Xcrsp34 antibody, crap34 antibody, crsp34 antibody, crsp8-B antibody, med27-a antibody, med27-b antibody, trap37 antibody, mediator complex subunit 27 antibody, Mediator complex subunit 27 antibody, cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa antibody, hypothetical protein antibody, mediator complex subunit 27 S homeolog antibody, MED27 antibody, med27 antibody, crsp8 antibody, Med27 antibody, med27.S antibody

Background:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene: MED27

Molecular Weight: 35 kDa

Gene ID: 9442

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha