P27 (AA 140-220) antibody
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- Target
- P27
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Binding Specificity
- AA 140-220
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- p27 is a protein encoded by the CDKN1B gene which is approximately 22 kDa. p27 is localised to the nucleus and cytoplasm. It is involved in CDK-mediated phosphorylation and removal of Cdc6, RET signalling, GAB1 signalosome and cell cycle role of SCF complex in cell cycle regulation. It is a cyclin-dependent kinase inhibitor that binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, which are important in controlling the cell cycle progression at G1. p27 is expressed in all tissues with the highest levels in skeletal muscles. Mutations in the CDKN1B gene may result in multiple endocrine neoplasia. STJ94866 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of p27 protein.
- Specificity
- P27 Polyclonal Antibody detects endogenous levels of p27 protein.
- Characteristics
- Rabbit polyclonal to p27.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human p27 around the non-phosphorylation site of T198.
- Isotype
- IgG
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- Application Notes
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WB 1:500-1:2000
ELISA 1:5000 - Comment
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Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- P27
- Synonyms
- Kip1 antibody, p27 antibody, cyclin dependent kinase inhibitor 1B antibody, CDKN1B antibody
- Target Type
- Viral Protein
- Molecular Weight
- 27 kDa
- Gene ID
- 1027
- UniProt
- P46527
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