PPP1CA antibody (pThr320)

Catalog No. ABIN6282675

Product Details anti-PPP1CA Antibody

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Binding Specificity: AA 260-340, pThr320

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1CA antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PP1α is a protein encoded by the PPP1CA gene which is approximately 37,5 kDa. PP1α is localised to the cytoplasm and nucleus. It is involved in vascular smooth muscle contraction, regulation of lipid metabolism and insulin signalling-generic cascades. It is one of the three catalytic subunits of protein phosphatase 1. It is an enzyme that catalyses the removal of phosphate groups from serine and/or threonine residues by hydrolysis of phosphoric acid monoesters. They oppose the action of kinases and phosphorylases and are involved in signal transduction. PP1α is expressed in the lung, muscle, blood, pancreas and liver. Increased activity of this protein has been observed in the end stage of heart failure. STJ91134 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous PP1α protein which only binds about T320 when T320 is phosphorylated.

Specificity: Phospho-PP1α (T320) Polyclonal Antibody detects endogenous levels of PP1α protein only when phosphorylated at T320.

Characteristics: Rabbit polyclonal to Phospho-PP1α (T320).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PP1alpha around the phosphorylation site of T320.

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:300
ELISA 1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PPP1CA

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Alternative Name: PP1alpha (PPP1CA Products)

Synonyms: PP-1A antibody, PP1A antibody, PP1alpha antibody, PPP1A antibody, Ppp1c antibody, dism2 antibody, ppp1a antibody, wu:fc04c08 antibody, wu:fc09b07 antibody, wu:fc30g11 antibody, wu:fe05h08 antibody, zgc:85729 antibody, Ppp1ca antibody, fb18b03 antibody, fd20h04 antibody, wu:fb18b03 antibody, wu:fd20h04 antibody, wu:fl22a03 antibody, zgc:55744 antibody, zgc:76940 antibody, protein phosphatase 1 catalytic subunit alpha antibody, protein phosphatase 1, catalytic subunit, alpha isoform antibody, protein phosphatase 1, catalytic subunit, alpha isozyme L homeolog antibody, protein phosphatase 1, catalytic subunit, alpha isozyme antibody, protein phosphatase 1, catalytic subunit, alpha isozyme a antibody, protein phosphatase 1, catalytic subunit, alpha isozyme b antibody, PPP1CA antibody, Ppp1ca antibody, ppp1ca.L antibody, ppp1ca antibody, ppp1caa antibody, ppp1cab antibody

Molecular Weight: 37 kDa

Gene ID: 5499

UniProt: P62136

Pathways: M Phase, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Lipid Metabolism