COL4a3 antibody (Internal Region)

Catalog No. ABIN6285019

Product Details anti-COL4a3 Antibody

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Binding Specificity: AA 770-850, Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4a3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal to COL4A3.

Specificity: COL4A3 Polyclonal Antibody detects endogenous levels of COL4A3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human COL4A3

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000

Comment:

Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for COL4a3

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Alternative Name: COL4A3 (COL4A3 Products)

Synonyms: zTumstatin antibody, [a]3(IV) antibody, alpha3(IV) antibody, collagen type IV alpha 3 chain antibody, collagen, type IV, alpha 3 antibody, COL4A3 antibody, col4a3 antibody, Col4a3 antibody

Molecular Weight: 140 kDa

Gene ID: 1285

UniProt: Q01955

Pathways: Sensory Perception of Sound, Positive Regulation of Endopeptidase Activity