PI3K p85/p55 antibody (pTyr199, pTyr467)

Catalog No. ABIN6288345

Product Details anti-PI3K p85/p55 Antibody

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Binding Specificity: AA 410-490, pTyr199, pTyr467

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3K p85/p55 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PI 3-kinase p85/p55 is a protein encoded by the PIK3R1 gene which is approximately 83,5 kDa. PI 3-kinase p85/p55 is localised to the cytoplasm, nucleus and cis-Golgi network. It is involved in RET signalling, IL-2 pathway, downstream signalling of activated FGFR2, regulation of lipid metabolism and insulin signalling-generic cascades. PI 3-Kinases are a family of lipid kinases capable of phosphorylating the 3'OH of the inositol ring of phosphoinositides. They are responsible for coordinating a diverse range of cell functions including proliferation and survival. PI 3-kinase p85/p55 is expressed in the nervous system, muscle, liver, heart and kidney. Mutations in the PIK3R1 gene may result in Agammaglobulinemia and SHORT syndrome. STJ90384 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous PI 3-kinase p85/p55 protein which only binds about Y467/199 when Y467/199 is phosphorylated.

Specificity: Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85/p55 protein only when phosphorylated at Y467/199.

Characteristics: Rabbit polyclonal to Phospho-PI 3-kinase p85/p55 (Y467/199).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000

Comment:

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PI3K p85/p55

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Alternative Name: PI 3-kinase p85/p55 (PI3K p85/p55 Products)

Molecular Weight: 54/83 kDa

Gene ID: 5295

UniProt: P27986