BAX antibody (N-Term)

Catalog No. ABIN6291820

Product Details anti-BAX Antibody

Target: BAX (BCL2-Associated X Protein (BAX))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Detects a band of approximately 21 kDa by Western blot in the following cell lysate: hCat, At-T20, MDA-MB-231, H69 and HUH.

Purification: This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Recombinant affinity purified peptide derived from within residues 90 aa to the N-terminus of human BAX produced in E. coli.

Isotype: IgG

Application Details

Application Notes: Western blot 1:500-1:5,000 Immunofluorescence 1:50-1:250 Immunohistochemistry (paraffin) ND Immunohistochemistry (frozen) ND

Comment:

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Restrictions: For Research Use only

Handling

Concentration: 4 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeated freeze-thaw cycles.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX (BAX Products)

Synonyms: BAX-ALPHA antibody, bax-A antibody, xBax antibody, xlbax antibody, BAX antibody, bax antibody, fj16e01 antibody, wu:fc50b10 antibody, wu:fj16e01 antibody, BCL2L4 antibody, zgc:112983 antibody, BCL2 associated X, apoptosis regulator antibody, BCL2-associated X protein L homeolog antibody, BCL2-associated X protein antibody, bcl2-associated X protein, a antibody, bcl2-associated X protein, b antibody, BAX antibody, bax.L antibody, bax antibody, baxa antibody, Bax antibody, baxb antibody

Background: Goat polyclonal antibody to BAX. This protein belongs to the BCL2 protein family that act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of BAX gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.

Molecular Weight: 22 kDa

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response