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CD133 antibody (Prominin 1) Primary Antibody

PROM1 Reactivity: Human FACS, IHC, WB Host: Mouse Monoclonal W6B3C1 unconjugated
Catalog No. ABIN6559814
$437.18
Plus shipping costs $45.00
0.1 mg
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  • Target
    CD133 (PROM1)
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This CD133 antibody is un-conjugated
    • 3
    • 2
    • 1
    Application
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    Specificity
    The mouse monoclonal antibody W6B3C1 recognizes a glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.
    HLDA VII Workshop
    Cross-Reactivity (Details)
    Human
    Purification
    Purified from hybridoma culture supernatant by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    WERI-RB-1 retinoblastoma cell line
    Clone
    W6B3C1
    Isotype
    IgG1
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    CD133 (PROM1)
    Alternative Name
    CD133 (PROM1 Antibody Abstract)
    Synonyms
    Prom, AC133, CD133, Prom-1, Proml1, 4932416E19Rik, CORD12, MCDR2, PROML1, RP41, STGD4, prominin 1, Prom1, PROM1
    Background
    CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
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