CD133 antibody (Prominin 1) (PE)

Details for Product anti-PROM1 Antibody No. ABIN6559834
Antigen
Reactivity
Human
4
Host
Mouse
4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD133 antibody is conjugated to PE
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
4
4
2
2
Options
Clone 293C3
Isotype IgG2b
Specificity The mouse monoclonal antibody 293C3 recognizes the epitope 2 on human CD133 (CD133/2), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of human renal progenitors.
HLDA VII Workshop
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD133 (PROM1 Antibody Abstract)
Background CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Prominin 1 (PROM1) antibody (PE) (ABIN6559834) Surface staining of CD133 in human peripheral blood with anti-CD133 (293C3) PE.