PCNA antibody (Proliferating Cell Nuclear Antigen)

Details for Product anti-PCNA Antibody No. ABIN6566543
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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260
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59
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40
39
33
32
22
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19
12
12
11
8
7
5
5
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2
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
288
230
4
2
Clonality
Polyclonal
Conjugate
This PCNA antibody is un-conjugated
24
19
9
8
8
8
8
8
8
4
4
4
4
4
4
2
2
2
2
2
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2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
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107
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72
53
34
33
18
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9
7
7
4
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3
1
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Options
Immunogen Recombinant protein of human PCNA
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name Proliferating Cell Nuclear Antigen (PCNA Antibody Abstract)
Background

Synonyms: ATLD2,cb16,Cyclin,DNA polymerase delta auxiliary protein,etID36690.10,fa28e03,fb36g03,HGCN8729,MGC8367,Mutagen-sensitive 209 protein,OTTHUMP00000030189,OTTHUMP00000030190,PCNA,Pcna/cyclin,PCNA,PCNAR,Polymerase delta accessory protein,Proliferating cell nuclear antigen,wu:fa28e03,wu:fb36g03

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Molecular Weight

Observed_MW: 35kDa

Calculated_MW: 28kDa

Gene ID 5111
UniProt P12004
Pathways Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-Proliferating Cell Nuclear Antigen (PCNA) antibody (ABIN6566543) Western blot analysis of extracts of various cell lines, using PCNA antibody.
Image no. 2 for anti-Proliferating Cell Nuclear Antigen (PCNA) antibody (ABIN6566543) Immunohistochemistry of paraffin-embedded human colon using PCNA antibody.
Image no. 3 for anti-Proliferating Cell Nuclear Antigen (PCNA) antibody (ABIN6566543) Immunohistochemistry of paraffin-embedded human stomach using PCNA antibody.