PHAX antibody (C-Term)

Catalog No. ABIN656761

Product Details anti-PHAX Antibody

Target: PHAX (phosphorylated Adaptor For RNA Export (PHAX))

Binding Specificity: AA 297-326, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PHAX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human PHAX.

Clone: RB32550

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: PHAX Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

Target Details for PHAX

Target: PHAX (phosphorylated Adaptor For RNA Export (PHAX))

Alternative Name: PHAX (PHAX Products)

Synonyms: RNUXA antibody, 2810055C14Rik antibody, 4933427L19Rik antibody, AU018701 antibody, AU018854 antibody, D18Ertd65e antibody, Rnuxa antibody, p55 antibody, phosphorylated adaptor for RNA export antibody, PHAX antibody, Phax antibody

Background: A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

Molecular Weight: 44403

Gene ID: 51808

NCBI Accession: NP_115553

UniProt: Q9H814

Pathways: Ribonucleoprotein Complex Subunit Organization