IQSEC1 antibody (AA 60-110)

Catalog No. ABIN5690018

Product Details anti-IQSEC1 Antibody

Target: IQSEC1 (IQ Motif and Sec7 Domain 1 (IQSEC1))

Binding Specificity: AA 60-110

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IQSEC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.

Purification: IQSEC1 antibody is affinity chromatography purified via peptide column.

Immunogen: IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1.

The immunogen is located within amino acids 60 - 110 of IQSEC1.

Isotype: IgG

Application Details

Application Notes: IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 2 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IQSEC1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: IQSEC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for IQSEC1

Target: IQSEC1 (IQ Motif and Sec7 Domain 1 (IQSEC1))

Alternative Name: IQSEC1 (IQSEC1 Products)

Synonyms: ARF-GEP100 antibody, ARFGEP100 antibody, BRAG2 antibody, GEP100 antibody, AW561907 antibody, BC026481 antibody, D6Ertd349e antibody, cI-43 antibody, mKIAA0763 antibody, IQ motif and Sec7 domain 1 antibody, IQSEC1 antibody, Iqsec1 antibody

Background: ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).

Molecular Weight: Predicted: 106 kDa

Observed: 103 kDa

Gene ID: 9922

NCBI Accession: NP_055684

UniProt: Q6DN90