MAPT antibody (pSer396)

Catalog No. ABIN196790

Product Details anti-MAPT Antibody

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Binding Specificity: pSer396

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Tau around the phosphorylation site of Serine 396 (Y-K-Sp-P-V).

Application Details

Application Notes: Western blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

References for anti-MAPT antibody (ABIN196790)

Thysiadis, Mpousis, Avramidis, Katsamakas, Balomenos, Remelli, Efthimiopoulos, Sarli: "Discovery of novel phenoxazinone derivatives as DKK1/LRP6 interaction inhibitors: Synthesis, biological evaluation and structure-activity relationships." in: Bioorganic & medicinal chemistry, Vol. 24, Issue 5, pp. 1014-22, (2016) (PubMed).

Mpousis, Thysiadis, Avramidis, Katsamakas, Efthimiopoulos, Sarli: "Synthesis and evaluation of gallocyanine dyes as potential agents for the treatment of Alzheimer's disease and related neurodegenerative tauopathies." in: European journal of medicinal chemistry, Vol. 108, pp. 28-38, (2016) (PubMed).

Eritja, Santacana, Maiques, Gonzalez-Tallada, Dolcet, Matias-Guiu: "Modeling glands with PTEN deficient cells and microscopic methods for assessing PTEN loss: endometrial cancer as a model." in: Methods (San Diego, Calif.), Vol. 77-78, pp. 31-40, (2015) (PubMed).

Jiang, Wang, Wang, Hawke, Zheng, Li, Zhou, Majumder, Bi, Liu, Huang, Lu: "PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells." in: Nature communications, Vol. 5, pp. 5566, (2015) (PubMed).

Bockmeyer, Kern, Forstmeier, Lovric, Modde, Agustian, Steffens, Birschmann, Traeder, Dämmrich, Schwarz, Kreipe, Bröcker, Becker: "Arteriolar vascular smooth muscle cell differentiation in benign nephrosclerosis." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 27, Issue 9, pp. 3493-501, (2013) (PubMed).

Gui, Han, Zhang, Liang, Wang, Xuan, Yu, Shang: "Dimerization of ZIP promotes its transcriptional repressive function and biological activity." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 886-95, (2012) (PubMed).

Lu, Berglund, Larsson, Höög, Farnebo, Bränström: "Calmodulin and calmodulin-dependent protein kinase II inhibit hormone secretion in human parathyroid adenoma." in: The Journal of endocrinology, Vol. 208, Issue 1, pp. 31-9, (2011) (PubMed).

Ishiguro, Ando, Maeda, Watanabe, Goto: "Cutting edge: tubulin ? functions as an adaptor in NFAT-importin ? interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 2710-3, (2011) (PubMed).

Godfrey, Anderson, Papanastasiou, Takeuchi, Boshoff: "Inhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA." in: Blood, Vol. 105, Issue 6, pp. 2510-8, (2005) (PubMed).

Dempsey, Sailor, Bowen, Türeyen, Vemuganti: "Stroke-induced progenitor cell proliferation in adult spontaneously hypertensive rat brain: effect of exogenous IGF-1 and GDNF." in: Journal of neurochemistry, Vol. 87, Issue 3, pp. 586-97, (2003) (PubMed).

Watanabe, Sugaya, Atkins, Aquilino, Yang, Borris, Brady, Blauvelt: "Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen prolongs the life span of primary human umbilical vein endothelial cells." in: Journal of virology, Vol. 77, Issue 11, pp. 6188-96, (2003) (PubMed).

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT / tau (MAPT Products)

Synonyms: DDPAC antibody, FTDP-17 antibody, MAPTL antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, AI413597 antibody, AW045860 antibody, Mtapt antibody, Tau antibody, RNPTAU antibody, pTau antibody, tau antibody, xtp antibody, MAPT antibody, PHF-tau antibody, slc6a6 antibody, taut antibody, wu:fc26e12 antibody, microtubule associated protein tau antibody, microtubule-associated protein tau antibody, microtubule associated protein tau S homeolog antibody, Microtubule-associated protein tau antibody, solute carrier family 6 (neurotransmitter transporter), member 6b antibody, MAPT antibody, Mapt antibody, mapt.S antibody, LOC5580230 antibody, CpipJ_CPIJ013260 antibody, tau antibody, slc6a6b antibody

Background: Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau

Gene ID: 9606

UniProt: P10636

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size