ALK antibody (AA 200-335)

Catalog No. ABIN6654205

Product Details anti-ALK Antibody

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Binding Specificity: AA 200-335

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALK antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Purity: Protein G affinity

Immunogen: A portion of amino acids 200-335 from the human protein was used as the immunogen for this ALK antibody.

Clone: ATKR-1

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the ALK antibody should be determined by the researcher.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the ALK antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ALK

Target: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Alternative Name: ALK / Anaplastic Lymphoma Kinase (ALK Products)

Synonyms: Tcrz antibody, CD246 antibody, NBLST3 antibody, ALK antibody, anaplastic lymphoma kinase antibody, anaplastic lymphoma receptor tyrosine kinase antibody, ALK receptor tyrosine kinase antibody, Alk antibody, ALK antibody

Background: Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. [UniProt]

Pathways: RTK Signaling