SMARCA5 antibody

Catalog No. ABIN6655221

Product Details anti-SMARCA5 Antibody

Target: SMARCA5 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 5 (SMARCA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMARCA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Purification: Anti-hSNF2H was clarified from mouse ascitic fluid and is specific for hSNF2H.

Immunogen:

Immunogen: Anti-hSNF2H Antibody was produced in mice by repeated immunization with a recombinant protein of human SNF2H.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: Anti-hSNF2H Antibody is suitable for Immunofluorescence, Immunoprecipitation and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 121.9 kDa in the appropriate cell lysate or extract.

Immunoprecipitation Dilution: 1 μg/IP

Western Blot Dilution: 1:1,000

IF Microscopy Dilution: 1:100 - 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for SMARCA5

Target: SMARCA5 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 5 (SMARCA5))

Alternative Name: hSNF2H (SMARCA5 Products)

Synonyms: fb26d12 antibody, fb49g04 antibody, chunp6878 antibody, im:7146484 antibody, wu:fb26d12 antibody, wu:fb49g04 antibody, zgc:158434 antibody, smarca5 antibody, iswi antibody, SMARCA5 antibody, hiswi antibody, snf2h antibody, hsnf2h antibody, wcrf135 antibody, MGC79455 antibody, ISWI antibody, SNF2H antibody, WCRF135 antibody, hISWI antibody, hSNF2H antibody, 4933427E24Rik antibody, D030040M08Rik antibody, D330027N15Rik antibody, MommeD4 antibody, Snf2h antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 S homeolog antibody, smarca5 antibody, smarca5.S antibody, SMARCA5 antibody, Smarca5 antibody

Background:

Synonyms: mouse anti-hSNF2H Antibody, mouse anti-SNF2H Antibody, mouse anti-Sucrose nonfermenting protein 2 homolog antibody, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5, Sucrose nonfermenting protein 2 homolog

Background: Complexes containing Snf2h (SMARCA5) are capable of forming ordered nucleosome arrays on chromatin in vitro (may require intact histone H4 tails). SNF2h is also required for replication of pericentric heterochromatin in S-phase, specifically in conjunction with BAZ1A. Snf2h probably also plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Snf2h is a catalytic subunit of the four known chromatin-remodeling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. Snf2h possesses intrinsic ATP-dependent nucleosome remodelling activity. Anti-hSNF2H Antibody is ideal for research in Chromatin Remodeling, Epigenetics and Gene Expression.

Gene Name: SMARCA5

Gene ID: 8467

NCBI Accession: NP_003592

UniProt: O60264

Pathways: Chromatin Binding