BCL3 antibody (C-Term)

Catalog No. ABIN6655290

Product Details anti-BCL3 Antibody

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Purification: This affinity purified antibody is directed against human BCL3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.

Immunogen:

Immunogen: Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-BCL-3 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at 47.5 kDa in western blot using appropriate lysates. Positive control used recombinant BCL3 protein, HEK293T overexpressing BCL3 or U87 whole cell lysates at 1 μg/mL in WB, and U87MG with PFA at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 5.0 μg/mL

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for BCL3

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Alternative Name: BCL3 (BCL3 Products)

Synonyms: BCL3 antibody, AI528691 antibody, Bcl-3 antibody, BCL4 antibody, D19S37 antibody, B-cell CLL/lymphoma 3 antibody, B cell leukemia/lymphoma 3 antibody, BCL3 antibody, Bcl3 antibody

Background:

Synonyms: rabbit anti-BCL3 antibody, rabbit anti-BCL-3 Antibody, BCL 3, B-cell lymphoma 3 protein, B-cell lymphoma 3 protein Antibody

Background: BCL3 belongs to the ANKRD family. The protein contains seven ankyrin repeats, which are closely related to those found in I kappa B proteins. BCL3 functions as a transcriptional co-activator, regulator of transcriptional activation, promotor of transcription, and inhibitor of nuclear translocation of NF-kappa B. It is a proto-oncogene identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. BCL3 contributes to the regulation of cell proliferation. Therefore, BCL3 may be associated with B-cell chronic lymphocytic leukemia, lymphoma, or other hematologic diseases. Anti-BCL3 Antibody is useful for researchers interested in Cancer Research, NF-Kappa B Pathway, and TNF Signaling Pathway.

Gene Name: BCL3

Gene ID: 602

NCBI Accession: NP_005169

UniProt: P20749

Pathways: NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus