TBPL1 antibody

Catalog No. ABIN6655376

Product Details anti-TBPL1 Antibody

Target: TBPL1 (TBP-Like 1 (TBPL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBPL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Purification: Anti-TRF2 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TRF2 from Human, Mouse, Muntjac (deer), and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TRF2 from other sources has not been determined.

Immunogen:

Immunogen: TRF2 Antibody was produced in mice by repeated immunizations with a full-length TRF2 human protein.

Immunogen Type: Recombinant Protein

Clone: 4A794-15

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-TRF2 Antibody is suitable for use in ChIP, ELISA, Flow (Intracellular) Cytometry, IF/ICC, IHC (frozen), IP, and WB. Expect a band approximately 55.6 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

ChIP Dilution: User Optimized

Immunoprecipitation Dilution: 2 μg/10^6 cells

ELISA Dilution: 2 μg/mL

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 2-4 μg/mL

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for TBPL1

Target: TBPL1 (TBP-Like 1 (TBPL1))

Alternative Name: TRF2 (TBPL1 Products)

Synonyms: MGC:8389 antibody, MGC:9620 antibody, STUD antibody, TLF antibody, TLP antibody, TRF2 antibody, tlf antibody, 4732475G08 antibody, AW011832 antibody, AW491032 antibody, D18347 antibody, STD antibody, TRP antibody, Tlp antibody, tlp antibody, trf antibody, trf2 antibody, trp antibody, zgc:92340 antibody, TATA binding protein 1 antibody, TFIID-1 antibody, TRANSCRIPTION FACTOR IID-1 antibody, TATA-box binding protein like 1 antibody, TATA-binding protein 1 antibody, TATA-box binding protein like 1 L homeolog antibody, TATA box binding protein-like 1 antibody, TBP-like 1 antibody, TATA binding protein 1 antibody, TBPL1 antibody, tbp1 antibody, tbpl1.L antibody, tbpl1 antibody, Tbpl1 antibody, TBP1 antibody

Background:

Synonyms: TRBF2, TRF2, Telomeric repeat-binding factor 2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

Background: TRF2 antibody detects human, mouse, deer and rat TRF2. TRF2 is an ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1, contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types. Anti TRF2 antibody is ideal for investigators involved in apoptosis and chromatin research.

Gene Name: TERF2

Gene ID: 7014

NCBI Accession: NP_005643

UniProt: Q15554