Mex-3 Homolog D (C. Elegans) (MEX3D) (Internal Region) antibody

Details for Product No. ABIN6655563
Antigen
Epitope
Internal Region
3
2
1
1
Reactivity
Human
9
Host
Rabbit
8
1
Clonality
Polyclonal
Application
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
9
6
4
2
2
1
Options
Immunogen

Immunogen: Anti-Rkhd1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the internal region of human Rkhd1.

Immunogen Type: Peptide

Isotype IgG
Cross-Reactivity (Details) Cross reactivity with Rkhd1 from other sources has not been determined.
Purification Anti-Rkhd1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Antigen
Alternative Name Rkhd1 (MEX3D Antibody Abstract)
Background

Synonyms: Rkhd1 Antibody, MEX3, TINO, RKHD1, MEX-3D, RNF193, OK/SW-cl.4, KIAA2031, RNA-binding protein MEX3D, RING finger and KH domain-containing protein 1

Background: Rkhd1, also known as TINO or MEX3D is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd1, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd1 binds to the AU-rich element in the 3'-untranslated region of Bcl-2 mRNA and is thought to be a negative regulator of Bcl-2 expression. Rkhd3 and Rkhd4, but not Rkhd1, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

Gene Name: MEX3D

Gene ID 399664
UniProt Q86XN8
Application Notes

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Rkhd1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 65 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000-1:20,000

Western Blot Dilution: 1-2 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Supplier Images
Image no. 1 for anti-Mex-3 Homolog D (C. Elegans) (MEX3D) (Internal Region) antibody (ABIN6655563) Western Blot of Rkhd1 Antibody Western Blot of Rkhd1 antibody. Lane A: MDA-MB-361 cel...
Image no. 2 for anti-Mex-3 Homolog D (C. Elegans) (MEX3D) (Internal Region) antibody (ABIN6655563) Immunohistochemistry of Rkhd1 Antibody Immunohistochemistry of Rkhd1 antibody. Tissue...
Image no. 3 for anti-Mex-3 Homolog D (C. Elegans) (MEX3D) (Internal Region) antibody (ABIN6655563) Immunofluorescence of Rkhd1 Antibody Immunofluorescence Microscopy of Rkhd1 antibody....