IL32 antibody (Interleukin 32) (C-Term)

Details for Product anti-IL32 Antibody No. ABIN6655809
Antigen
Epitope
C-Term
22
16
10
8
7
6
5
5
3
3
2
2
2
2
2
1
1
1
1
Reactivity
Human
135
23
4
Host
Rabbit
115
19
1
Clonality
Polyclonal
Conjugate
This IL32 antibody is un-conjugated
7
6
5
4
4
3
1
1
1
1
1
1
1
Application
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
103
61
43
19
12
7
6
5
4
3
2
2
1
1
1
Options
Immunogen

Immunogen: Anti-IL-32 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the C-terminus of human IL-32.

Immunogen Type: Peptide

Isotype IgG
Cross-Reactivity Human, Mouse (Murine)
Purification Anti-IL-32 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody detects all isoforms of IL-32.
Antigen
Alternative Name IL-32 (IL32 Antibody Abstract)
Background

Synonyms: IL-32 Antibody, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, NK4, Interleukin-32, Natural killer cells protein 4, IL-32

Background: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-a, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFN-gamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.

Gene Name: IL32

Gene ID 9235
UniProt P24001
Application Notes

Immunohistochemistry Dilution: 10 μg/mL

Application Note: Anti-IL-32 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 26 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:50,000-1:100,000

Western Blot Dilution: 5-10 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Supplier Images
Image no. 1 for anti-Interleukin 32 (IL32) (C-Term) antibody (ABIN6655809) Western Blot of IL-32 Antibody - Western Blot of IL-32 antibody in human spleen lysat...
Image no. 2 for anti-Interleukin 32 (IL32) (C-Term) antibody (ABIN6655809) Immunofluorescence of IL-32 Antibody Immunofluorescence Microscopy of IL-32 antibody....
Image no. 3 for anti-Interleukin 32 (IL32) (C-Term) antibody (ABIN6655809) Immunohistochemistry of IL-32 Antibody - Immunohistochemistry of IL-32 antibody.   Ti...