Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1) (Internal Region) antibody

Details for Product No. ABIN6655890
Target
Binding Specificity
Internal Region
8
8
7
7
2
2
2
1
1
1
1
Reactivity
Human
56
23
19
Host
Rabbit
45
7
5
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
36
30
13
7
6
5
1
1
1
Options
Immunogen

Immunogen: Anti-NADE antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 14 amino acids near the internal region of human NADE.

Immunogen Type: Peptide

Isotype IgG
Cross-Reactivity Human, Mouse (Murine)
Cross-Reactivity (Details) Cross reactivity with NADE from other sources has not been determined.
Purification Anti-NADE Antibody is purified by ion exchange chromatography.
Target
Alternative Name NADE (NGFRAP1 Antibody Abstract)
Background

Synonyms: NADE Antibody, Bex, BEX3, NADE, HGR74, DXS6984E, Protein BEX3, Brain-expressed X-linked protein 3

Background: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

Gene Name: NGFRAP1

Gene ID 27018
NCBI Accession NP_996798
UniProt Q00994
Pathways Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity
Application Notes

Immunohistochemistry Dilution: 2 μg/mL

Application Note: Anti-NADE Antibody has been tested for use in ELISA, Western Blotting, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 13 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1 μg/mL

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Images
Image no. 1 for anti-Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1) (Internal Region) antibody (ABIN6655890) Western Blot of NADE Antibody - Western Blot of NADE antibody in human brain cell lys...
Image no. 2 for anti-Nerve Growth Factor Receptor (TNFRSF16) Associated Protein 1 (NGFRAP1) (Internal Region) antibody (ABIN6655890) Immunohistochemistry of NADE Antibody - Immunohistochemistry of NADE antibody. Tissue...