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Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (N-Term) Primary Antibody

CASP4 Reactivity: Human ELISA, FM, IHC, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6655931
$379.00
Plus shipping costs $45.00 and $24.00 dry ice
100 μg
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  • Target
    Caspase 4 (CASP4)
    Binding Specificity
    • 11
    • 10
    • 10
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 81
    • 35
    • 18
    • 15
    • 2
    Human
    Host
    • 92
    • 11
    • 7
    • 3
    • 1
    Rabbit
    Clonality
    • 99
    • 14
    Polyclonal
    Conjugate
    • 64
    • 18
    • 11
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Caspase 4 antibody is un-conjugated
    Application
    • 101
    • 43
    • 38
    • 11
    • 10
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
    Cross-Reactivity
    Human, Mouse (Murine)
    Purification
    Anti-Caspase-4 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Depending on cell lines or tissues used, other cleavage products may be observed.
    Immunogen

    Immunogen: Anti-Caspase-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-4.

    Immunogen Type: Peptide

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 2 μg/mL

    Application Note: Anti-Caspase-4 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa in Western Blots of specific cell lysates and tissues.

    ELISA Dilution: 1:5000-1:10000

    Western Blot Dilution: 0.5-1 μg/mL

    IF Microscopy Dilution: 10 μg/mL

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    Caspase 4 (CASP4)
    Alternative Name
    Caspase-4 (CASP4 Antibody Abstract)
    Synonyms
    ICE(rel)II, ICEREL-II, ICH-2, Mih1/TX, TX, Casp11, CASP13, CASP1, CASP4, Caspl, ich-3, Casp4, caspase 4, caspase 4, apoptosis-related cysteine peptidase, caspase-4, CASP4, Casp4, LOC100731032
    Background

    Synonyms: Caspase-4 Antibody, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, ICH2, Caspase-4, Protease ICH-2, CASP-4

    Background: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1b and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.

    Gene Name: CASP4

    Gene ID
    837
    UniProt
    P49662
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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