Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (N-Term) Primary Antibody
- Caspase 4 (CASP4)
- This Caspase 4 antibody is un-conjugated
- ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
- Human, Mouse (Murine)
- Anti-Caspase-4 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Depending on cell lines or tissues used, other cleavage products may be observed.
Immunogen: Anti-Caspase-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-4.
Immunogen Type: Peptide
- Application Notes
Immunohistochemistry Dilution: 2 μg/mL
Application Note: Anti-Caspase-4 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa in Western Blots of specific cell lysates and tissues.
ELISA Dilution: 1:5000-1:10000
Western Blot Dilution: 0.5-1 μg/mL
IF Microscopy Dilution: 10 μg/mL
- For Research Use only
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Caspase 4 (CASP4)
- Alternative Name
- Caspase-4 (CASP4 Antibody Abstract)
- ICE(rel)II, ICEREL-II, ICH-2, Mih1/TX, TX, Casp11, CASP13, CASP1, CASP4, Caspl, ich-3, Casp4, caspase 4, caspase 4, apoptosis-related cysteine peptidase, caspase-4, CASP4, Casp4, LOC100731032
Synonyms: Caspase-4 Antibody, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, ICH2, Caspase-4, Protease ICH-2, CASP-4
Background: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1b and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.
Gene Name: CASP4
- Gene ID
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity