UHRF1 Binding Protein 1 (UHRF1BP1) (Internal Region) antibody Primary Antibody
- Binding Specificity
- Internal Region
- ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
- Human, Mouse (Murine)
- Anti-UHRF1BP1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. UHRF1BP1 antibody is predicted to not cross-react with UHRF1BP1L.
Immunogen: Anti-UHRF1BP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the internal region of human UHRF1BP1.
Immunogen Type: Peptide
- Application Notes
Immunohistochemistry Dilution: 2.5 μg/mL
Application Note: Anti-UHRF1BP1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 160 kDa in Western Blots of specific cell lysates and tissues.
ELISA Dilution: 1:10,000-1:20,000
Western Blot Dilution: 1-2 μg/mL
IF Microscopy Dilution: 20 μg/mL
- For Research Use only
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Alternative Name
- UHRF1BP1 (UHRF1BP1 Antibody Abstract)
Synonyms: UHRF1BP1 Antibody, ICBP90, C6orf107, dJ349A12.1, UHRF1-binding protein 1, ICBP90-binding protein 1
Background: The Ubiquitin-like containing PHD and RING finger domains 1-binding protein 1 (UHRF1BP1), also known as ICBP90, is a transcription and cell cycle regulator. It specifically binds to the histone H3 N-terminal tail when methylated on K9 by two functional domains, a PHD finger that defines the binding specificity and an SRA (SET- and RING-associated) domain that promotes binding activity. UHRF1BP1 is required for proper heterochromatin formation in mammalian cells. Furthermore, UHRF1BP1 is thought to be a pivotal target for the ERK1/2 signaling pathway to control the proliferation of Jurkat T cells.
Gene Name: UHRF1BP1
- Gene ID
- NCBI Accession