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Geminin Coiled-Coil Domain Containing (GMNC) (Internal Region) antibody Primary Antibody

GMNC Reactivity: Human ELISA, WB Host: Rabbit Polyclonal
Catalog No. ABIN6656036
$379.00
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100 μg
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  • Target
    Binding Specificity
    Internal Region
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    ELISA, Western Blotting (WB)
    Cross-Reactivity
    Human, Mouse (Murine)
    Cross-Reactivity (Details)
    Cross reactivity with GEMC1 from other sources has not been determined.
    Purification
    Anti-GEMC1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Immunogen

    Immunogen: Anti-GEMC1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide near the internal region of human GEMC1.

    Immunogen Type: Peptide

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-GEMC1 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 38 kDa in Western Blots of specific cell lysates and tissues.

    ELISA Dilution: 1:10,000

    Western Blot Dilution: 1 μg/mL

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    Alternative Name
    GEMC1 (GMNC Antibody Abstract)
    Synonyms
    GEMC1, Gemc1, Gm1778, Gm606, gemc1, xGEMC1, geminin coiled-coil domain containing, geminin coiled-coil domain containing S homeolog, GMNC, Gmnc, gmnc.S
    Background

    Synonyms: GEMC1 Antibody, GEMC1, GEMC1, Geminin coiled-coil domain-containing protein 1

    Background: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.

    Gene Name: GEMC1

    Gene ID
    647309
    NCBI Accession
    NP_001140158
    UniProt
    A6NCL1
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