SCARB2 antibody (Internal Region)

Catalog No. ABIN6656141

Product Details anti-SCARB2 Antibody

Target: SCARB2 (Scavenger Receptor Class B, Member 2 (SCARB2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCARB2 antibody is un-conjugated

Application: ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with LIMP2 from other sources has not been determined.

Purification: Anti-LIMP2 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: Anti-LIMP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human LIMP2.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-LIMP2 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 55 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for SCARB2

Target: SCARB2 (Scavenger Receptor Class B, Member 2 (SCARB2))

Alternative Name: LIMP2 (SCARB2 Products)

Synonyms: cd36l2 antibody, fi13c07 antibody, chunp6914 antibody, wu:fi13c07 antibody, SCARB2 antibody, scarb2 antibody, AMRF antibody, CD36L2 antibody, EPM4 antibody, HLGP85 antibody, LGP85 antibody, LIMP-2 antibody, LIMPII antibody, SR-BII antibody, 9330185J12Rik antibody, Cd36l2 antibody, MLGP85 antibody, LimpII antibody, scavenger receptor class B, member 2a antibody, scavenger receptor class B member 2 antibody, scavenger receptor class B, member 2 antibody, scarb2a antibody, SCARB2 antibody, scarb2 antibody, Scarb2 antibody

Background:

Synonyms: LIMP2 Antibody, AMRF, EPM4, LGP85, CD36L2, HLGP85, LIMP-2, LIMPII, SR-BII, LIMP2, Lysosome membrane protein 2, 85 kDa lysosomal membrane sialoglycoprotein

Background: The lysosomal integral membrane protein 2 (LIMP2) is a heavily glycosylated type III transmembrane protein, the majority of which exists in the lumen of the lysosome and a cytoplasmic domain of approximately 20 amino acids. A deficiency of LIMP2 in mice causes uretic pelvic junction obstruction, deafness, and peripheral neuropathy associated with impaired vesicular trafficking and distribution of apically expressed proteins. More recently, LIMP2 was shown to act as a receptor to bind beta-glucocerebrosidase, the enzyme defective in Gaucher disease, a lysosomal storage disorder. LIMP2-deficient mice showed missorted as well as secreted beta-glucocerebrosidase, suggesting that LIMP2 also functions as the mannose-6-phosphate-independent trafficking receptor.

Gene Name: SCARB2

Gene ID: 950

UniProt: Q14108