Nanos Homolog 1 antibody (Internal Region)

Catalog No. ABIN6656281

Product Details anti-Nanos Homolog 1 Antibody

Target: Nanos Homolog 1 (NANOS1)

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanos Homolog 1 antibody is un-conjugated

Application: ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with Nanos1 from other sources has not been determined.

Purification: Anti-Nanos1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: Anti-Nanos1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from near the internal region of human Nanos1.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Nanos1 Antibody has been tested for use in ELISA, Western Blotting and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 30 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000-1:20,000

Western Blot Dilution: 1-2 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Nanos Homolog 1

Target: Nanos Homolog 1 (NANOS1)

Alternative Name: Nanos1 (NANOS1 Products)

Synonyms: NOS1 antibody, nanos1 antibody, fb76c12 antibody, wu:fb93h11 antibody, wu:fb98e07 antibody, wu:fq21a10 antibody, Xcat-2 antibody, nos1 antibody, xnos1 antibody, xcat-2 antibody, LOC100144352 antibody, NANOS1A antibody, nanos C2HC-type zinc finger 1 antibody, nanos homolog 1 antibody, nanos homolog 1 (Drosophila) antibody, nanos homolog 1 L homeolog antibody, Nanos homolog 1 antibody, nanos1 antibody, NANOS1 antibody, nanos1 antibody, Nanos1 antibody, nanos1.L antibody, nano1 antibody, nanos1a antibody

Background:

Synonyms: Nanos1 Antibody, NOS1, SPGF12, NOS1, Nanos homolog 1, NOS-1

Background: Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.

Gene Name: NANOS1

Gene ID: 340719

UniProt: Q8WY41

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction