Olfactomedin 1 antibody (Internal Region)

Catalog No. ABIN6656290

Product Details anti-Olfactomedin 1 Antibody

Target: Olfactomedin 1 (OLFM1)

Binding Specificity: Internal Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Olfactomedin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Pancortin Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Pancortin from Mouse, Human, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with Pancortin from other sources has not been determined. Neuroscience research.

Immunogen:

Immunogen: Pancortin Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to an internal region of human pancortin-1.

Immunogen Type: Peptide

Clone: S96-7

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-Pancortin Antibody is suitable for use in WB, IHC and IP. Expect a band approximately ~70 kDa (Pancortin-1, Pancortin-3) and ~20 kDa (Pancortin-2 and Pancortin-4) on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Olfactomedin 1

Target: Olfactomedin 1 (OLFM1)

Alternative Name: Pancortin (OLFM1 Products)

Background:

Synonyms: Noelin, AMY, Noe1, Olfm1, Olfactomedin1, Neuronal olfactomedin-related ER localized protein, Pancortin

Background: Pancortin, a member of a diverse family of secreted glycoproteins, is implicated in the development of the nervous system, and the glaucoma-associated protein myocilin. Studies suggest that Pancortin-2 interact with WAVE1 in a novel cell death cascade in adult, but not embryonic, cerebral cortical neurons. Specifically pancortin-2 is a mediator of ischemia-induced apoptosis of neurons in the adult cerebral cortex, and functions in a novel mitrochondrial/actin associated protein complex that sequesters Bcl-xL. It is also involved in the generation of neural crest cells, and in neurogenesis.

Gene Name: Olfm1

Gene ID: 56177

NCBI Accession: NP_001033701

UniProt: O88998