CISD2 antibody (CDGSH Iron Sulfur Domain 2) (Internal Region) Primary Antibody
- Binding Specificity
- Internal Region
- This CISD2 antibody is un-conjugated
- ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
- Human, Mouse (Murine), Rat (Rattus)
- Cross-Reactivity (Details)
- Cross reactivity with CISD2 from other sources has not been determined.
- Anti-CISD2 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Immunogen: Anti-CISD2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the internal region of human CISD2.
Immunogen Type: Peptide
- Application Notes
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-CISD2 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 15 kDa in Western Blots of specific cell lysates and tissues.
ELISA Dilution: User Optimized
Western Blot Dilution: User Optimized
IF Microscopy Dilution: User Optimized
- For Research Use only
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Alternative Name
- CISD2 (CISD2 Antibody Abstract)
Synonyms: CISD2 Antibody, ERIS, WFS2, ZCD2, NAF-1, Miner1, CDGSH2, ERIS, CDGSH iron-sulfur domain-containing protein 2, Endoplasmic reticulum intermembrane small protein
Background: Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.
Gene Name: CISD2
- Gene ID
- NCBI Accession
- Activation of Innate immune Response