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RUN and SH3 Domain Containing 1 (RUSC1) (C-Term) antibody Primary Antibody

RUSC1 Reactivity: Human ELISA, WB Host: Rabbit Polyclonal
Catalog No. ABIN6657109
$379.00
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100 μg
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  • Target
    Binding Specificity
    C-Term
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    ELISA, Western Blotting (WB)
    Cross-Reactivity
    Human, Mouse (Murine), Rat (Rattus)
    Cross-Reactivity (Details)
    Cross reactivity with RUSC1 from other sources has not been determined.
    Purification
    Anti-RUSC1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Immunogen

    Immunogen: Anti-RUSC1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the C-terminus of human RUSC1.

    Immunogen Type: Peptide

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-RUSC1 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 96 kDa in Western Blots of specific cell lysates and tissues.

    ELISA Dilution: 1:10,000

    Western Blot Dilution: 1 μg/mL

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    Alternative Name
    RUSC1 (RUSC1 Antibody Abstract)
    Synonyms
    NESCA, 2210403N08Rik, AA408288, RUN and SH3 domain containing 1, RUSC1, Rusc1
    Background

    Synonyms: RUSC1 Antibody, NESCA, NESCA, RUN and SH3 domain-containing protein 1, New molecule containing SH3 at the carboxy-terminus, Nesca

    Background: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30 % amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IkB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-kB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-kB activation pathway.

    Gene Name: RUSC1

    Gene ID
    1489668
    NCBI Accession
    NP_001098673
    UniProt
    P59594
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