Phosphatidylinositol Binding clathrin Assembly Protein (PICALM) (N-Term) antibody Primary Antibody
- Binding Specificity
- ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
- Human, Mouse (Murine), Rat (Rattus)
- Cross-Reactivity (Details)
- Cross reactivity with PICALM from other sources has not been determined.
- Anti-PICALM Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Immunogen: Anti-PICALM antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the N-terminus of human PICALM.
Immunogen Type: Peptide
- Application Notes
Application Note: Anti-PICALM Antibody has been tested for use in ELISA, Western Blotting and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 71 kDa in Western Blots of specific cell lysates and tissues.
ELISA Dilution: 1:10,000
Western Blot Dilution: 1 μg/mL
IF Microscopy Dilution: 20 μg/mL
- For Research Use only
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Alternative Name
- PICALM (PICALM Antibody Abstract)
Synonyms: PICALM Antibody, LAP, CALM, CLTH, Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein
Background: PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).
Gene Name: PICALM
- Gene ID
- NCBI Accession
- Synaptic Membrane