Albumin antibody (Internal Region)

Catalog No. ABIN6657317

Product Details anti-Albumin Antibody

Target: Albumin (ALB)

Binding Specificity: Internal Region

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This Albumin antibody is un-conjugated

Application: ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with Albumin from other sources has not been determined.

Purification: Anti-Albumin Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Albumin antibody was prepared from chicken egg fractions produced by repeated immunizations with a 13 amino acid synthetic peptide near the internal region of human Albumin.

Immunogen Type: Peptide

Isotype: IgY

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Albumin Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 69 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000-1:20,000

Western Blot Dilution: 1 - 2 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-Albumin antibody (ABIN6657317)

Haas, Onstead-Haas, Kurban, Shah, Plazarte, Chamseddin, Mooradian: "High-Throughput Analysis Identifying Drugs That Regulate Apolipoprotein A-I Synthesis." in: Assay and drug development technologies, Vol. 15, Issue 8, pp. 362-371, (2018) (PubMed).

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin (ALB Products)

Synonyms: PRO0883 antibody, PRO0903 antibody, PRO1341 antibody, ALB antibody, CSA antibody, Alb-1 antibody, Alb1 antibody, Albza antibody, LOC100136344 antibody, alb-a antibody, alb-b antibody, albumin antibody, serum albumin 1 antibody, albumin S homeolog antibody, ALB antibody, Alb antibody, LOC100136344 antibody, alb.S antibody

Background:

Synonyms: Albumin Antibody, PRO0883, PRO0903, PRO1341, Serum albumin

Background: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The resulting product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. At least two isoforms of Albumin are known to exist.

Gene Name: ALB

Gene ID: 213

UniProt: P02768

Pathways: Lipid Metabolism