TRPM7 antibody (C-Term)

Catalog No. ABIN6657588

Product Details anti-TRPM7 Antibody

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRPM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human

Purification: Anti-TRPM7 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with TRPM7 from Human, Rat, and Mouse based on 100% homology with the immunizing sequence. No cross-reactivity against TrpM6. Cross-reactivity with TRPM7 from other sources has not been determined. Channels and Transporters research.

Immunogen:

Immunogen: TRPM7 Antibody was produced in mice by repeated immunizations raised against a fusion proteins corresponding to the c-terminus region of mouse TrpM7.

Immunogen Type: Peptide

Clone: S74-25

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1000

Application Note: Anti-TRPM7 Antibody is suitable for use in WB, IHC, and IF microscopy. Expect a band approximately ~220 kDa on specific lysates, in extracts of cells transiently transfected with TrpM7. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for TRPM7

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Alternative Name: TRPM7 (TRPM7 Products)

Synonyms: cb495 antibody, TRPM7 antibody, chak antibody, chak1 antibody, ltrpc7 antibody, trp-plik antibody, ALSPDC antibody, CHAK antibody, CHAK1 antibody, LTRPC7 antibody, TRP-PLIK antibody, 2310022G15Rik antibody, 4833414K03Rik antibody, 5033407O22Rik antibody, LTrpC-7 antibody, Ltpr7 antibody, Ltrpc7 antibody, TRPPLIK antibody, Chak antibody, Trp-plik antibody, transient receptor potential cation channel subfamily M member 7 antibody, transient receptor potential cation channel, subfamily M, member 7 antibody, TRPM7 antibody, trpm7 antibody, Trpm7 antibody

Background:

Synonyms: CHAK antibody, CHAK1 antibody, LTRPC7 antibody, TRP PLIK antibody, Channel kinase 1 antibody, Transient receptor potential cation channel subfamily M member 7 antibody, Channel-kinase 1 antibody, Long transient receptor potential channel 7 antibody, LTrpC-7 antibody, Transient receptor potential-phospholipase C-interacting kinase antibody, TRP-PLIK antibody, Trpm7 antibody

Background: TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

Gene Name: Trpm7

Gene ID: 58800

NCBI Accession: NP_001157797

UniProt: Q923J1