KCNMB3 antibody (N-Term)

Catalog No. ABIN6657686

Product Details anti-KCNMB3 Antibody

Target: KCNMB3 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M beta Member 3 (KCNMB3))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNMB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-BK channel BETA3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with BK channel BETA3 from human, mouse, and rat based on 100% homology with the immunizing sequence. No cross-reactivity against BKBeta1, BKBeta1, BKBeta3b or BKBeta4. Cross-reactivity with BK channel BETA3 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: BK channel BETA3 Antibody was produced in mice by repeated immunizations with a fusion protein (N-terminus region) of mouse BKBeta3.

Immunogen Type: Recombinant Protein

Clone: S40B-18

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-BK channel Beta3 Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~32 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for KCNMB3

Target: KCNMB3 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M beta Member 3 (KCNMB3))

Alternative Name: BK channel BETA3 (KCNMB3 Products)

Synonyms: KCNMB3 antibody, BKBETA3 antibody, HBETA3 antibody, KCNMB2 antibody, KCNMBL antibody, SLOBETA3 antibody, EG435726 antibody, Gm5707 antibody, potassium calcium-activated channel subfamily M regulatory beta subunit 3 antibody, potassium channel subfamily M regulatory beta subunit 3 S homeolog antibody, potassium large conductance calcium-activated channel, subfamily M beta member 3 antibody, potassium large conductance calcium-activated channel, subfamily M, beta member 3 antibody, KCNMB3 antibody, kcnmb3.S antibody, Kcnmb3 antibody

Background:

Synonyms: HBETA3, KCNMB2, KCNMBL, BKBeta3, KCNMB3, Calcium-activated potassium channel subunit beta-3, BK channel subunit beta-3, BKbeta3, Hbeta3, Calcium-activated potassium channel, subfamily M subunit beta-3 Charybdotoxin receptor subunit beta-3, K(VCA)beta-3, Maxi K channel subunit beta-3, Slo-beta-3

Background: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release. A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia. This has major implications on other physiological processes in tissues other than the brain.

Gene Name: KCNMB3

Gene ID: 27094

NCBI Accession: NP_055222

UniProt: Q9NPA1