CASK antibody

Catalog No. ABIN6658140

Product Details anti-CASK Antibody

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CASK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)

Purification: Anti-CASK Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with CASK from Mouse, Rat, Human, Zebrafish, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with CASK from other sources has not been determined. Scaffolds research.

Immunogen:

Immunogen: CASK Antibody was produced in mice by repeated immunizations with a fusion protein of rat CASK/Lin2.

Immunogen Type: Recombinant Protein

Clone: S56A-50

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CASK Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~100 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for CASK

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Alternative Name: CASK (CASK Products)

Synonyms: CAKI antibody, CAMGUK antibody, CG13412 antibody, CG13413 antibody, CG6703 antibody, CMG antibody, CMG/CASK antibody, Caki antibody, CamGUK antibody, CamguK antibody, Camguk antibody, Cask antibody, Cmg antibody, DLin-2 antibody, Dmel\\CG6703 antibody, RE09582p antibody, caki antibody, camguk antibody, cmg antibody, dCASK antibody, wu:fb70g06 antibody, CAGH39 antibody, FGS4 antibody, LIN2 antibody, MICPCH antibody, MRXSNA antibody, TNRC8 antibody, DXPri1 antibody, DXRib1 antibody, LIN-2 antibody, Pals3 antibody, mLin-2 antibody, CG6703 gene product from transcript CG6703-RF antibody, Peripheral plasma membrane protein CASK antibody, CAMK/CASK protein kinase antibody, peripheral plasma membrane protein CASK antibody, calcium/calmodulin-dependent serine protein kinase b antibody, calcium/calmodulin-dependent serine protein kinase a antibody, calcium/calmodulin dependent serine protein kinase antibody, calcium/calmodulin-dependent serine protein kinase (MAGUK family) antibody, calcium/calmodulin-dependent serine protein kinase (MAGUK family) S homeolog antibody, CASK antibody, Bm1_33725 antibody, LOAG_17286 antibody, Tsp_03763 antibody, caskb antibody, caska antibody, Cask antibody, cask.S antibody

Background:

Synonyms: Peripheral plasma membrane protein CASK, Calcium/calmodulin-dependent serine protein kinase

Background: CASK (calmodulin sensitive kinase) is a ~112 kDa member of the membrane associated quanylate kinase (MAGUK) protein family. It is an adaptor protein with a calcium/calmodulin-dependent protein kinase domain, a SH3 domain, aguanylate kinase homology domain (GUK) and a PDZ domain. CASK links transmembrane proteins to the cytoskeleton and signaling molecules. In particular, CASK binds to Neurexin to stabilize pre- and post-synaptic structrures. While most of CASK proteins are cytoplasmic, a portion of the protein enters the nucleus, where it acts as a transcriptional co-activator. Manifesting the importance of CASK, transgenic mice with insertional mutations die within 24 hours of births. CASK is also localized in the nuclei of basal keratinocytes in newborn rodent skin and developing hair follicles. It may play a role in wound healing.

Gene Name: Cask

Gene ID: 29647

NCBI Accession: NP_071520

UniProt: Q62915

Pathways: Synaptic Vesicle Exocytosis