GABRA2 antibody (Cytoplasmic Loop)

Catalog No. ABIN6658231

Product Details anti-GABRA2 Antibody

Target: GABRA2 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 2 (GABRA2))

Binding Specificity: Cytoplasmic Loop

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity to GABA(A) from other species has not been determined.

Purification: Anti-GABA(A) Receptor alpha 2 antibody is directed against rat GABA(A) receptor alpha 2 protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Labeling is absent in α2-subunit knockout animals. Reactivity is expected from bovine, canine, human, non-human primate, Zebrafish.

Immunogen:

Immunogen: Anti-GABA(A) Receptor alpha 2 Antibody was produced in rabbit by repeated immunizations with a fusion protein from the cytoplasmic loop of the rat receptor alpha 2 subunit.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-GABA(A) Receptor alpha 2 (Rabbit) Antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 2 subunit of the GABA(A) receptor in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

References for anti-GABRA2 antibody (ABIN6658231)

Ohkawa, Satake, Yokoi, Miyazaki, Ohshita, Sobue, Takashima, Watanabe, Fukata, Fukata: "Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 24, pp. 8151-63, (2014) (PubMed).

Target Details for GABRA2

Target: GABRA2 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 2 (GABRA2))

Alternative Name: GABA(A) Receptor alpha 2 (GABRA2 Products)

Synonyms: C630048P16Rik antibody, Gabra-2 antibody, gamma-aminobutyric acid type A receptor alpha2 subunit antibody, gamma-aminobutyric acid type A receptor alpha1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 2 antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 2 S homeolog antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 antibody, GABRA2 antibody, GABRA1 antibody, gabra2 antibody, Gabra2 antibody, gabra2.S antibody

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2

Background: GABA(A) Receptor alpha 2 Antibody detects GABA(A) receptor alpha 2 protein. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor. GABA(A) receptor alpha 2 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRA2

Gene ID: 61856

NCBI Accession: NP_001129251

UniProt: P23576