GABRA5 antibody (Cytoplasmic Loop)

Catalog No. ABIN6658274

Product Details anti-GABRA5 Antibody

Target: GABRA5 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 5 (GABRA5))

Binding Specificity: Cytoplasmic Loop

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA5 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Anti-GABA(A) Receptor alpha 5 Antibody is directed against rat GABA(A) Receptor alpha 5. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity is expected from the following species based on 100% sequence homology: bovine, chicken, canine, human, mouse, non-human primate, and zebra fish.

Immunogen:

Immunogen: Anti-GABA(A) Receptor alpha 5 Antibody was produced by repeated immunizations with recombinant fusion proteins from the cytoplasmic loop of the alpha 5 subunit.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-GABA(A) Receptor alpha 5 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to the alpha 5 subunit of the GABA(A) receptor in Western Blots of rat brain extracts.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for GABRA5

Target: GABRA5 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 5 (GABRA5))

Alternative Name: GABA(A) Receptor alpha 5 (GABRA5 Products)

Synonyms: GABRA5 antibody, si:dkey-93p24.1 antibody, A230018I05Rik antibody, gamma-aminobutyric acid type A receptor alpha5 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 5 antibody, gamma-aminobutyric acid type A receptor alpha 5 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5 antibody, gamma-aminobutyric acid receptor subunit alpha-5 antibody, GABRA5 antibody, gabra5 antibody, Gabra5 antibody, LOC100653497 antibody

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5, Gabra5

Background: Anti-GABA(A) Receptor alpha 5 Antibody detects GABA(A) Receptor alpha 4. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. GABA(A) receptor alpha 5 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRA5

Gene ID: 29707

NCBI Accession: NP_058991

UniProt: P19969

Pathways: Sensory Perception of Sound, Synaptic Membrane