RANKL antibody (AA 221-300)

Catalog No. ABIN668556

Product Details anti-RANKL Antibody

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Binding Specificity: AA 221-300

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse OPG

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-RANKL antibody (ABIN668556)

Kerschan-Schindl, Schramek, Butylina, Föger-Samwald, Pietschmann: "Differential Expression of Dickkopf 1 and Periostin in Mouse Strains with High and Low Bone Mass." in: Biology, Vol. 11, Issue 12, (2022) (PubMed).

Zhan, Liu, Wang: "The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." in: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 536-546, (2018) (PubMed).

Yu, Gong, Lin, Wang, Liu, Li: "Denervation effectively aggravates rat experimental periodontitis." in: Journal of periodontal research, Vol. 52, Issue 6, pp. 1011-1020, (2017) (PubMed).

Zhang, Chen, Wei, Liu, Lei, Bai: "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway." in: Chemico-biological interactions, Vol. 256, pp. 188-97, (2016) (PubMed).

Chen, Xue, Shen, Mu, Fu: "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway." in: International journal of molecular medicine, Vol. 37, Issue 2, pp. 329-38, (2016) (PubMed).

He, Wang, Wang, Tian, Jiang, Ren, Qiu, Fu: "Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1054-60, (2016) (PubMed).

Xiao, Hu, Li, Li: "Bone fracture healing is delayed in splenectomic rats." in: Life sciences, Vol. 173, pp. 55-61, (2016) (PubMed).

Zhang, Liu, Li, Bai: "Mycobacterium tuberculosis 10-kDa co-chaperonin regulates the expression levels of receptor activator of nuclear factor-?B ligand and osteoprotegerin in human osteoblasts." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 919-924, (2015) (PubMed).

Yu, Lv, Zhang, Zhang, Xiao, Li: "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." in: Journal of molecular histology, Vol. 46, Issue 2, pp. 195-203, (2015) (PubMed).

Herath, Lion, Klein, Stenger, Scheuer, Holstein, Mörsdorf, Rollmann, Pohlemann, Menger, Histing: "Stimulation of angiogenesis by cilostazol accelerates fracture healing in mice." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, (2015) (PubMed).

Li, Wu, Ma, Hao, Chen, Fu, Chen, Wang: "Oral administration of 5-hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats." in: Archives of oral biology, Vol. 60, Issue 5, pp. 789-98, (2015) (PubMed).

Yang, Li, Yang, Shen, Zou, Zhu, Zhang, Yang, Li: "Efficacy and safety of echinacoside in a rat osteopenia model." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 926928, (2013) (PubMed).

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: OPG (TNFSF11 Products)

Background:

Synonyms: Opg, TR1, OCIF, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, Tnfrsf11b

Background: Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF1/TRAIL and protect against apoptosis. TNFSF1/TRAIL binding blocks the inhibition of osteoclastogenesis.

Gene ID: 18383

UniProt: O08712

Pathways: NF-kappaB Signaling