ATF3 antibody (AA 51-150)

Catalog No. ABIN669561

Product Details anti-ATF3 Antibody

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Binding Specificity: AA 51-150

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATF3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ATF3 antibody (ABIN669561)

Smith, Seto, Vivekanandan-Giri, Yuan, Playford, Manna, Hasni, Kuai, Mehta, Schwendeman, Pennathur, Kaplan et al.: "Lupus high-density lipoprotein induces proinflammatory responses in macrophages by binding lectin-like oxidised low-density lipoprotein receptor 1 and failing to promote activating transcription ..." in: Annals of the rheumatic diseases, (2016) (PubMed).

Aguirre, López-Alonso, González-López, Amado-Rodríguez, Batalla-Solís, Astudillo, Blázquez-Prieto, Fernández, Galván, dos Santos, Albaiceta: "Defective autophagy impairs ATF3 activity and worsens lung injury during endotoxemia." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 6, pp. 665-76, (2014) (PubMed).

Yuan, Han, Cong, Ge, Ma, Dai, Li, Bi: "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." in: International journal of nanomedicine, Vol. 9, pp. 4829-46, (2014) (PubMed).

Hu, Li, Li: "MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1." in: PLoS ONE, Vol. 8, Issue 6, pp. e68291, (2013) (PubMed).

Feng, Sun, Wu, Lu, Qu, Sun, Tian, Zhang, Li, Liu: "Upregulation of ATF-3 is correlated with prognosis and proliferation of laryngeal cancer by regulating Cyclin D1 expression." in: International journal of clinical and experimental pathology, Vol. 6, Issue 10, pp. 2064-70, (2013) (PubMed).

Target Details for ATF3

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Alternative Name: ATF3 (ATF3 Products)

Synonyms: zgc:55526 antibody, ATF3 antibody, LRG-21 antibody, LRF-1 antibody, LRFI antibody, atf-3 antibody, activating transcription factor 3 antibody, activating transcription factor 3 L homeolog antibody, atf3 antibody, ATF3 antibody, Atf3 antibody, atf3.L antibody

Background:

Synonyms: Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3

Background: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Gene ID: 467

UniProt: P18847

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response