Rabbit anti-Mouse (Murine) IgG (Chain gamma 1), (Chain gamma 2a), (Chain gamma 2b), (Chain gamma 3) Antibody (DyLight 405) - Preadsorbed

Details for Product No. ABIN6698971
Antigen
Epitope
Chain gamma 1, Chain gamma 2a, Chain gamma 2b, Chain gamma 3
1571
134
100
56
34
33
25
5
5
5
5
5
4
4
4
3
2
1
1
1
Reactivity
Mouse (Murine)
354
342
337
233
232
122
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
846
798
336
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
DyLight 405
236
220
209
150
147
130
75
49
48
35
30
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogen

Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Immunogen Type: Native Protein

Isotype IgG
Characteristics

Synonyms: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody DyLight™ 405 conjugation, rabbit anti-Mouse IgG DyLight™405 conjugated Antibody

Background: Anti-Mouse IgG DyLight405 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification Preadsorption: Solid phase absorption
Labeling Ratio 2.0
Antigen
Application Notes

Application Note: Mouse IgG subclass pan reactive Secondary Antibody is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Rabbit anti-mouse IgG antibody DyLight™ 405 is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Mouse IgG secondary antibody is available in a variety of formats.

FLISA Dilution: >1:20,000

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiry Date 12 months
Product cited in: Zu, Wang, Ping, Sun: "Tan IIA inhibits H1299 cell viability through the MDM4‑IAP3 signaling pathway." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 2384-2392, 2018 (PubMed).