Rabbit anti-Mouse IgG3 (Chain gamma 3) Antibody (DyLight 549) - Preadsorbed

Catalog No. ABIN6699079

Product Details

Target: IgG3

Binding Specificity: Chain gamma 3

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: DyLight 549

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Characteristics:

Synonyms: Rabbit Anti-Mouse IgG3 (Gamma 3 chain) DyLight 549™ Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody DyLight 549™ Conjugation

Background: Anti-Mouse IgG3 DyLight 549 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG3 heavy chain

Isotype: IgG

Labeling Ratio: 3.1

Application Details

Application Notes:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Baumann, Bakkers, Buettner, Hartmann, Grove, Langereis, de Groot, Mühlenhoff: "9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate." in: Nature communications, Vol. 6, pp. 7673, (2016) (PubMed).

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody