GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (N-Term)

Details for Product anti-GAPDH Antibody No. ABIN6699202
Antigen
  • G3PD
  • GAPD
  • Gapd
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • cb609
  • gapd
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • KNC-NDS6
  • g3pd
  • G3PDH
  • glyceraldehyde-3-phosphate dehydrogenase
  • Glyceraldehyde 3 phosphate dehydrogenase 1
  • glyceraldehyde-3-phosphate dehydrogenase S homeolog
  • glyceraldehyde-3-phosphate dehydrogenase, type I
  • olfactory receptor 8K3
  • GAPDH
  • Gapdh
  • Gapdh1
  • gapdh
  • gapdh.S
  • gapDH
  • LOC100404960
  • LOC614985
Epitope
N-Term
73
32
13
12
8
8
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
571
275
254
120
82
81
60
59
52
44
36
31
30
28
26
24
23
17
16
9
9
8
7
7
6
6
6
6
6
5
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
376
221
36
26
17
1
Clonality
Polyclonal
Conjugate
This GAPDH antibody is un-conjugated
49
34
20
7
7
6
5
5
5
5
5
5
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
600
265
165
159
91
84
56
27
25
25
19
15
13
7
6
6
4
2
2
2
1
1
1
Options
Immunogen

Immunogen: GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).

Isotype IgG
Cross-Reactivity Chicken, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
Purification Anti-GAPDH Antibody is directed against human GAPDH protein.  The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast.  Broad reactivity makes this antibody an excellent loading control.
Plasmids, Primers & others Plasmids, Primers & others GAPDH products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background

Synonyms: Loading Control Antibody, Anti-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

Background: GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. A loading control antibody is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience.

Gene Name: GAPDH

Gene ID 7669492
UniProt P04406
Application Notes

Immunohistochemistry Dilution: 1:100 - 1:1,000

Application Note: Anti-GAPDH Antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation.  Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract.  GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker.

ELISA Dilution: 1:10,000 - 1:40,000

Western Blot Dilution: 1:1,000 - 1:5,000

IF Microscopy Dilution: 1:100 - 1:1,000

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store Anti-GAPDH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Supplier Images
Image no. 1 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (N-Term) antibody (ABIN6699202) ELISA - GAPDH Antibody ELISA of Rabbit Anti-GAPDH Antibody. Antigen: HCT-116 cell lin...
Image no. 2 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (N-Term) antibody (ABIN6699202) Anti-GAPDH Antibody - Western Blot. Western blot using Affinity Purified anti-GAPDH ...
Image no. 3 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (N-Term) antibody (ABIN6699202) Anti-GAPDH Antibody - Immunohistochemistry Immunohistochemical staining using Affini...
Product cited in: Gonzales, De La Chapa, Saikumar, Singha, Dybdal-Hargreaves, Chavez, Horning, Parra, Kirma: "Co-targeting ALK and EGFR parallel signaling in oral squamous cell carcinoma." in: Oral oncology, Vol. 59, pp. 12-19, 2018 (PubMed).

Maxwell, Castro, Sutherland, Vaughan, Szarowicz, de Cabo, Mattison, Valdez: "α-Motor neurons are spared from aging while their synaptic inputs degenerate in monkeys and mice." in: Aging cell, Vol. 17, Issue 2, 2018 (PubMed).

Roy, Jerman, Jozsef, McNamara, Onyekaba, Sun, Marin: "Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation." in: The Journal of biological chemistry, Vol. 292, Issue 43, pp. 17703-17717, 2017 (PubMed).

Lum, Attridge, Morona: "Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection." in: PLoS ONE, Vol. 8, Issue 12, pp. e84975, 2014 (PubMed).

Piras, OConnor, French: "Systemic delivery of shRNA by AAV9 provides highly efficient knockdown of ubiquitously expressed GFP in mouse heart, but not liver." in: PLoS ONE, Vol. 8, Issue 9, pp. e75894, 2014 (PubMed).

Konkalmatt, Wang, Piras, Xu, OConnor, Beyers, Epstein, Annex, Hossack, French et al.: "Adeno-associated virus serotype 9 administered systemically after reperfusion preferentially targets cardiomyocytes in the infarct border zone with pharmacodynamics suitable for the attenuation of ..." in: The journal of gene medicine, Vol. 14, Issue 9-10, pp. 609-20, 2013 (PubMed).

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