Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 115-170) antibody Primary Antibody

NCF4 Reactivity: Human, Mouse, Rat IHC, WB Host: Rabbit Polyclonal unconjugated
Pubmed (1)
Catalog No. ABIN6712099
$350.00
Plus shipping costs $45.00
100 μL
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
  • Target
    Neutrophil Cytosolic Factor 4, 40kDa (NCF4)
    Binding Specificity
    AA 115-170
    • 17
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Reactivity
    Human, Mouse, Rat
    • 65
    • 41
    • 25
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    Host
    Rabbit
    • 55
    • 7
    • 6
    Clonality
    Polyclonal
    • 65
    • 3
    Conjugate
    Un-conjugated
    • 39
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    Immunohistochemistry (IHC), Western Blotting (WB)
    • 43
    • 20
    • 18
    • 13
    • 8
    • 4
    • 3
    • 2
    • 2
    Specificity
    P40 phox (R149) polyclonal antibody detects endogenous levels of p40 phox protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Purity
    > 95% (by SDS-PAGE)
    Immunogen
    Synthetic peptide, corresponding to amino acids 115-170 of Human p40-phox.
  • Application Notes
    WB: 1:500~1:1000 IHC: 1:50~1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    1 mg/mL in Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C short term. Aliquot and store at -20°C long term.
  • Wang, Yang, Han, Li, Jiang, Tian, Du, Li: "NADPH oxidases mediate a cellular "memory" of angiotensin II stress in hypertensive cardiac hypertrophy." in: Free radical biology & medicine, Vol. 65, pp. 897-907, 2015 (PubMed).

  • Target
    Neutrophil Cytosolic Factor 4, 40kDa (NCF4)
    Alternative Name
    p40-phox (NCF4 Antibody Abstract)
    Synonyms
    p40phox, AI451400, NCF, P40PHOX, SH3PXD4, neutrophil cytosolic factor 4, ncf4, Ncf4, NCF4
    Background
    Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation,including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase.
    Molecular Weight
    ~ 39 kDa
    UniProt
    Q15080
You are here:
help Support