YWHAZ antibody (Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide) (AA 200-250)

Details for Product anti-YWHAZ Antibody No. ABIN6712288
Antigen
  • 14-3-3-zeta
  • KCIP-1
  • YWHAD
  • 14-3-3zeta
  • Ywhaz
  • ACYPI003154
  • 14-3-3z
  • kcip-1
  • ywhaq
  • 1433z
  • ywhaz
  • ywhazb
  • 1110013I11Rik
  • AI596267
  • AL022924
  • AU020854
  • ywhaza
  • fb14h09
  • wu:fb05g08
  • wu:fb14h09
  • ywhai
  • zgc:55807
  • 14-3-3
  • 14-3-3 zeta
  • 14-3-3ZETA
  • 14-3-3leo
  • 2G1
  • 4-3-3 zeta
  • 5.11
  • 549
  • BEST:GH05075
  • CG17870
  • D14-3-3
  • D14-3-3zeta
  • Dmel\\CG17870
  • K
  • LEO
  • Leo
  • PAR-5
  • PAR5
  • Par-5
  • d14-3-3zeta
  • l(2)07103
  • l(2)46CFe
  • l(2)46Ee
  • leo
  • par-5
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
  • 14-3-3 protein zeta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta L homeolog
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta S homeolog
  • 14-3-3 protein zeta/delta pseudogene
  • CG17870 gene product from transcript CG17870-RE
  • YWHAZ
  • 14-3-3zeta
  • ywhaz
  • 1433z
  • ywhaz.L
  • Ywhaz
  • ywhaz.S
  • LOC100855903
Epitope
AA 200-250
58
12
11
10
9
9
7
6
6
6
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
239
171
144
24
23
19
19
11
7
6
6
6
4
3
2
2
1
1
Host
Rabbit
232
11
Clonality
Polyclonal
Conjugate
This YWHAZ antibody is un-conjugated
10
5
5
4
4
4
4
4
4
3
3
3
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
203
118
90
58
54
24
17
13
12
6
3
3
3
Options
Immunogen Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 zeta.
Specificity 14-3-3 ζ (W228) polyclonal antibody detects endogenous levels of 14-3-3 protein zeta/delta.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity > 95% (by SDS-PAGE)
Plasmids, Primers & others Plasmids, Primers & others YWHAZ products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name 14-3-3 zeta (YWHAZ Antibody Abstract)
Background 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
Molecular Weight ~ 28 kDa
UniProt P63104
Pathways Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1 mg/mL in Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term.
Supplier Images
Image no. 1 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (AA 200-250) antibody (ABIN6712288) Western blot (WB) analysis of 14-3-3 ζ (W228) pAb at 1:500 dilution Lane1:K562 whole ...
Product cited in: Nagappan, Park, Park, Kim, Hong, Kang, Zhang, Kim, Lee, Won, Kim: "Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells." in: BMC biochemistry, Vol. 14, pp. 24, 2014 (PubMed).

Nagappan, Park, Park, Hong, Yumnam, Lee, Kim, Kim, Lee, Lee, Cho, Lee, Won, Cho, Kim: "Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo-/- mice." in: Chemico-biological interactions, Vol. 206, Issue 2, pp. 143-52, 2013 (PubMed).

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