AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308)

Details for Product anti-AKT1 Antibody No. ABIN6713951
Antigen
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Alternatives
anti-Rat (Rattus) AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
Epitope
pThr308
184
93
88
74
52
50
45
30
30
26
21
18
18
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2
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2
2
2
2
2
2
2
2
2
2
2
2
2
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2
1
1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
1337
751
627
57
57
54
35
33
31
29
6
6
6
5
4
3
3
3
2
1
1
1
Host
Rabbit
1118
257
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
64
38
33
32
29
26
19
19
19
19
18
18
4
4
4
2
2
2
2
2
2
2
2
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2
2
2
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1
1
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1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
1121
608
605
230
170
143
136
64
55
38
32
18
13
11
8
5
3
3
2
1
1
1
1
1
Options
Immunogen Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308.
Specificity P-AKT (T308) polyclonal antibody detects endogenous levels of AKT1 only when phosphorylated at Thr308. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity > 95% (by SDS-PAGE)
Plasmids, Primers & others Plasmids, Primers & others AKT products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Molecular Weight ~ 60 kDa
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1 mg/mL in Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term.
Product cited in: Fang, Gu, Zhou, Liang, Ren, Liu, Zeng, Wu, Zhao, Huang, Zhang, Yi: "β-Catenin overexpression is associated with gefitinib resistance in non-small cell lung cancer cells." in: Pulmonary pharmacology & therapeutics, Vol. 28, Issue 1, pp. 41-8, 2015 (PubMed).

Guo, Zhang, Gao, Parry, Liu, Liu, Wang: "Quercetin and quercetin-3-O-glucuronide are equally effective in ameliorating endothelial insulin resistance through inhibition of reactive oxygen species-associated inflammation." in: Molecular nutrition & food research, Vol. 57, Issue 6, pp. 1037-45, 2014 (PubMed).

Gao, Liu, Huang, Zhang, Guo, Wang, Liu: "Positive and negative regulation of insulin action by genistein in the endothelium." in: The Journal of nutritional biochemistry, Vol. 24, Issue 1, pp. 222-30, 2013 (PubMed).

Fu, Wang, Guo, Xu, Wu: "XJP-1 protects endothelial cells from oxidized low-density lipoprotein-induced apoptosis by inhibiting NADPH oxidase subunit expression and modulating the PI3K/Akt/eNOS pathway." in: Vascular pharmacology, Vol. 58, Issue 1-2, pp. 78-86, 2013 (PubMed).

Tong, Lu, Wei, Wang, Xia, Dai et al.: "Gleditsioside B, a triterpene saponin isolated from the anomalous fruits of Gleditsia sinensis Lam., abrogates bFGF-induced endothelial cell migration through preventing the activation of MMP-2 and ..." in: Vascular pharmacology, Vol. 58, Issue 1-2, pp. 118-26, 2013 (PubMed).

Lu, Bian, Zhang, Zhang, Liu, Yan, Zhang, Jiang, Wei, Zhu, Chen, Wang, Chen, Yang, Liu, Li: "Interferon regulatory factor 3 is a negative regulator of pathological cardiac hypertrophy." in: Basic research in cardiology, Vol. 108, Issue 2, pp. 326, 2013 (PubMed).

Pan, Ge, Xu, Jia, Liu, Li, Liu: "The expression of chitinase 3-like 1: a novel prognostic predictor for hepatocellular carcinoma." in: Journal of cancer research and clinical oncology, Vol. 139, Issue 6, pp. 1043-54, 2013 (PubMed).

Wu, Qi, Liu, Cheng, Liu, Li, Wang et al.: "Mechanisms underlying protective effects of trimetazidine on endothelial progenitor cells biological functions against H2O2-induced injury: involvement of antioxidation and Akt/eNOS signaling ..." in: European journal of pharmacology, Vol. 707, Issue 1-3, pp. 87-94, 2013 (PubMed).

Cao, Cai, Cai, Tu: "Effect of 5-hydroxymethylfurfural derived from processed Cornus officinalis on the prevention of high glucose-induced oxidative stress in human umbilical vein endothelial cells and its mechanism." in: Food chemistry, Vol. 140, Issue 1-2, pp. 273-9, 2013 (PubMed).

Li, Yan, Zhang, Jiang, Sun, Hu, Sun, Xu: "miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6." in: FEBS letters, Vol. 587, Issue 12, pp. 1754-61, 2013 (PubMed).

Zeng, Li, Dong, Ma, Jiang, Jin, Tu: "Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis." in: European journal of pharmacology, Vol. 714, Issue 1-3, pp. 261-73, 2013 (PubMed).

Yao, Li, Xiong, Li, Jiang, Chen: "A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells." in: Cell and tissue research, Vol. 353, Issue 3, pp. 409-23, 2013 (PubMed).

Xing, Qi, Deng, Wang, Zhang, Chen: "Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition." in: Experimental cell research, Vol. 319, Issue 13, pp. 2058-72, 2013 (PubMed).

Liu, Luo, Zhang, Wang, Kou, Liu, Huang: "Modified Si-Miao-San extract inhibits inflammatory response and modulates insulin sensitivity in hepatocytes through an IKKβ/IRS-1/Akt-dependent pathway." in: Journal of ethnopharmacology, Vol. 136, Issue 3, pp. 473-9, 2012 (PubMed).

Pan, Gao, Lu, Xu, Xia, Dai: "Prevention of FGF-2-induced angiogenesis by scopoletin, a coumarin compound isolated from Erycibe obtusifolia Benth, and its mechanism of action." in: International immunopharmacology, Vol. 11, Issue 12, pp. 2007-16, 2012 (PubMed).

Xiao, Moon, Yan, Nian, Zhang, Liu, Lu, Guan, Chen, Jiang, Jiang, Liu, Li: "Cellular FLICE-inhibitory protein protects against cardiac remodelling after myocardial infarction." in: Basic research in cardiology, Vol. 107, Issue 1, pp. 239, 2012 (PubMed).

Shi, Yao, Liu, Wang, Lv, Zhang, Ji, Xu, He, Shi, Hou: "Highly frequent PIK3CA amplification is associated with poor prognosis in gastric cancer." in: BMC cancer, Vol. 12, pp. 50, 2012 (PubMed).

Huang, Liu, Du, Kou, Liu: "Puerarin attenuates endothelial insulin resistance through inhibition of inflammatory response in an IKKβ/IRS-1-dependent manner." in: Biochimie, Vol. 94, Issue 5, pp. 1143-50, 2012 (PubMed).

Wang, Gu, He, Ye, Chen, Zhang, Hai: "Glucose oxidase induces insulin resistance via influencing multiple targets in vitro and in vivo: The central role of oxidative stress." in: Biochimie, Vol. 94, Issue 8, pp. 1705-17, 2012 (PubMed).

Liu, Kou, Yu: "Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-κB activation." in: Neurochemistry international, Vol. 58, Issue 1, pp. 119-25, 2011 (PubMed).

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