CDC45 antibody (Cell Division Cycle 45 Homolog (S. Cerevisiae))

Details for Product anti-CDC45 Antibody No. ABIN6717713
Antigen
  • cdc45l
  • cdc45l2
  • porc-pi-1
  • CDC45L
  • CDC45L2
  • PORC-PI-1
  • Cdc45l
  • cell division cycle 45
  • cell division cycle 45 S homeolog
  • cell division cycle 45 L homeolog
  • cdc45
  • CDC45
  • cdc45.S
  • Cdc45
  • cdc45.L
Reactivity
Human, Mouse (Murine)
96
49
45
2
2
2
2
1
1
1
Host
Rabbit
85
12
Clonality
Polyclonal
Conjugate
This CDC45 antibody is un-conjugated
5
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
73
34
34
23
13
10
7
4
3
3
2
1
1
Options
Immunogen Recombinant full length Human cdc45.
Specificity Cdc45 polyclonal antibody detects endogenous levels of cdc45 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity > 95% (by SDS-PAGE)
Plasmids, Primers & others Plasmids, Primers & others CDC45 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name cdc45 (CDC45 Antibody Abstract)
Background The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Molecular Weight ~64 kDa
UniProt O75419
Pathways Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
Application Notes WB: 1:500~1:2000 IHC: 1:50~1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1 mg/mL in PBS with 0.1 %  Sodium Azide, 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term.
Supplier Images
Image no. 1 for anti-Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) antibody (ABIN6717713) Western blot analysis of cdc45 polyclonal antibody
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