ADO antibody (AA 49-261)

Catalog No. ABIN7354747

Product Details anti-ADO Antibody

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Binding Specificity: AA 49-261

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADO antibody is un-conjugated

Application: Flow Cytometry (FACS)

Purpose: Rabbit IgG Polyclonal Anti-Human ADO Antibody DyLight® 550 Conjugated, Flow Validated.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG Polyclonal Anti-Human ADO Antibody DyLight® 550 Conjugated, Flow Validated.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotype: IgG

Application Details

Application Notes:

Application details: Flow Cytometry|1-3 μg/1x106 cells

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: At 2-8°C for one year. Protect from light. Do not freeze.

Target Details for ADO

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Alternative Name: ADO (ADO Products)

Synonyms: C10orf22 antibody, Gm237 antibody, RGD1308233 antibody, wu:fc32g01 antibody, wu:fc52c12 antibody, zgc:101580 antibody, 2-aminoethanethiol dioxygenase antibody, 2-aminoethanethiol (cysteamine) dioxygenase antibody, 2-aminoethanethiol (cysteamine) dioxygenase a antibody, ADO antibody, Ado antibody, LOC100351598 antibody, adoa antibody

Background:

Synonyms: 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase, ADO, C10orf22

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.