RMI2 antibody

Catalog No. ABIN6719656

Product Details anti-RMI2 Antibody

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RMI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for RMI2 detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KMTDLSDNPI HESMWELEVE DLHRNIP

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for RMI2 detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence of human RMI2(KMTDLSDNPIHESMWELEVEDLHRNIP).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for RMI2

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Alternative Name: RMI2 (RMI2 Products)

Synonyms: BLAP18 antibody, C16orf75 antibody, C25H16orf75 antibody, C14H16orf75 antibody, A630055G03Rik antibody, Gm537 antibody, Gm929 antibody, RecQ mediated genome instability 2 antibody, RMI2 antibody, Rmi2 antibody

Background:

Synonyms: RecQ-mediated genome instability protein 2, hRMI2, BLM-associated protein of 18 kDa, BLAP18, RMI2, C16orf75

Background: RMI2 is a component of the BLM (RECQL3) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability. This gene is mapped to 16p13.13. RMI1 and RMI2 were present in approximately stoichiometric amounts with other BLM complex components, including topoisomerase-3-alpha (TOP3A), RPA (see RPA1), and BLAP250. RMI2 also associated with RMI1 and TOP3A in a second complex. RMI1 and RMI2 interacted directly, and both were essential for stability of the BLM complex. Depletion of either RMI1 or RMI2 depleted the other protein by 80 to 90 % . Chicken DT40 cells depleted of Rmi2 displayed elevated sister chromatid exchange, but other functions of the BLM complex appeared intact. Mutation analysis revealed that interaction between human RMI2 and BLM was essential for suppression of sister chromatid exchange.

Gene ID: 116028

UniProt: Q96E14

Pathways: DNA Damage Repair