Spindlin 1 antibody (AA 3-100)

Catalog No. ABIN672351

Product Details anti-Spindlin 1 Antibody

Target: Spindlin 1 (SPIN1)

Binding Specificity: AA 3-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spindlin 1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Specificity: There is 60 % sequence similarity with Spindlin 3, but the sequence is not continuous so the chance of bindin is low.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Spindin 1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Spindlin 1

Target: Spindlin 1 (SPIN1)

Alternative Name: Spindlin 1 (SPIN1 Products)

Synonyms: SPIN antibody, AA553322 antibody, AA673430 antibody, Spin antibody, spin antibody, spindlin antibody, MGC115436 antibody, Spindlin-1 antibody, DKFZp459K085 antibody, spindlin 1 antibody, spindlin 1 L homeolog antibody, SPIN1 antibody, Spin1 antibody, spin1 antibody, spin1.L antibody

Background:

Synonyms: SPIN, Spindlin-1, Ovarian cancer-related protein, Spindlin1, SPIN1, OCR

Background: Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway dowstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed:24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed:21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.

Gene ID: 10927

UniProt: Q9Y657